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    The tube was capped and shaken vertically for 5 min at 1500 strokes/min with an automatic pulverizer (Automill, Tokken). A total of 12 tubes could be processed in parallel. After a brief centrifugation, the bullet was removed with tweezers and the tube centrifuged again for 2 min.

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    A stainless-steel bullet (Tokken, Kashiwa, Japan), 20 μL of 1 μg/mL 3-amino-1-phenylbutane in water and 230 μL of water (for identification, no 3-amino-1-phenylbutane and 250 μL of water) were added. The tube was capped and shaken vertically for 5 min at 1500 strokes/min in an automatic pulverizer (Automill, Tokken).

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    All samples were stored at room temperature until the time of DNA extraction. Seed samples were ground for 1 min at 1500 strokes per min with an automatic pulverizer (Automill TK-AM5, Tokken

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    The tube was capped and shaken vertically for 5 min at 1500 strokes/min using an Automill automatic pulverizer (Tokken). After a brief centrifugation, the bullet was removed using tweezers and the suspension was filtered using a centrifugal filter unit fitted with a 0.45-μm polyvinylidene fluoride microporous membrane (Ultrafree MC, Millipore

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    The container was capped and shaken for 10 min at 25 Hz with a pulverizer (Automill, Tokken). The powdered hair sample (10 mg) was precisely weighed into a glass tube (10 mL volume). The internal standard mixture (5 μL) and 1 mL of 45% ammonium phosphate (PB, pH 8.4 adjusted with aqueous ammonia) were added. The solution was left to stand for

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    The ratios of NADPH/NADP + in the GBM cell lines were calculated using the NADP/NADPH Quantification Kit (BioVision, Mountain View, CA, USA) according to the manufacturer’s instructions. In brief, cell pellets were lysed in extraction buffer with vigorous shaking in an automatic homogenizer (Automill™; Tokken

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